Influence of gold nanoparticles on human fibroblast before and after cryopreservation

Скорочена інформація про документ
dc.contributor.authorPavlovich, EV
dc.contributor.authorVolkova, NA
dc.date.accessioned2022-09-12T10:17:13Z
dc.date.available2022-09-12T10:17:13Z
dc.date.issued2015
dc.description.abstractThe aim of our research was to study the influence of gold nanoparticles (AuNPs) on morphological and functional characteristics of the human fibroblast cells before and after cryopreservation. The effect of AuNPs on the viability, proliferative capacity, and apoptosis/necrosis processes of human fibroblast cells was studied. A stimulating effect of AuNPs at concentration of 6 μg/mL on the proliferative ability of fibroblast cells was shown. The results obtained by flow cytometry suggested that AuNPs at concentrations of 1.5 and 3 μg/mL by culturing for 7 days do not cause the development of necrosis and apoptosis in fibroblast cells. After 7 days culturing of the fibroblasts, cryopreserved in the presence of AuNPs at concentration of 6 μg/mL, a decrease in the percentage of viable cells and an increase of the number of apoptotic cells compared with the control samples were observed.uk_UA
dc.identifier.citationPavlovich, E.V., Volkova, N.A. (2015). Influence of Gold Nanoparticles on Human Fibroblast Before and After Cryopreservation. In: Fesenko, O., Yatsenko, L. (eds) Nanoplasmonics, Nano-Optics, Nanocomposites, and Surface Studies. Springer Proceedings in Physics, vol 167. Springer, Cham. https://doi.org/10.1007/978-3-319-18543-9_28uk_UA
dc.identifier.urihttp://cryo.net.ua/xmlui/handle/123456789/503
dc.language.isoenuk_UA
dc.publisherSpringer International Publishinguk_UA
dc.subjectCells Culture Fibroblasts Gold nanoparticles Apoptosis Necrosis Cytofluorimetry Culturing Adhesion Proliferation In vitro Light microscopy 7-AAD Annexin Stimulation Inhibition Suspensionuk_UA
dc.titleInfluence of gold nanoparticles on human fibroblast before and after cryopreservationuk_UA
dc.typeBook chapteruk_UA

Файли

Контейнер файлів

Зараз показуємо 1 - 1 з 1
Ескіз
Назва:
Nanoplasmonics2CNano-Optics2CNanocomposi1.pdf
Розмір:
16,91 MB
Формат:
Adobe Portable Document Format
Опис:
Завантажити

Ліцензійна угода

Зараз показуємо 1 - 1 з 1
Ескіз
Назва:
license.txt
Розмір:
1,69 KB
Формат:
Item-specific license agreed upon to submission
Опис:
Завантажити

Колекції

Відділ кріобіології системи репродукції