Discovery of Osmotic Responses of Sweet Potato Meristems in Cryoprotective Solution

Abstract

PVS2, PVSN cryoprotective solutions and sample exposure time of 40 and 60 min, respectively, are promising to develop the effective protocols for cryopreservation of sweet potato meristems. To establish procedures for cryopreservation of meristems of different plant species, it is necessary to study their osmotic response to cryoprotective solutions, that will allow to determine the optimal time of dehydration of experimental samples prior to cooling down to liquid nitrogen temperatures.