Чутливість еритроцитів людини і барана до гіпертонічного шоку залежно від температури, рН середовища та присутності алканолів

Abstract

Встановлено, що еритроцити барана більш чутливі до змін температури і менш чутливі до змін рН середовища при дії такого стресового чинника як гіпертонічний шок, ніж еритроцити людини, що зумовлено різним білковим і фосфоліпідним складом мембран еритроцитів людини і барана. Показано, що бутанол, на відміну від гексанолу, захищає еритроцити барана та людини в умовах гіпертонічного шоку. Захисний ефект бутанолу по відношенню до еритроцитів барана не залежить від рН середовища, а по відношенню до еритроцитів людини спостерігається при нефізіологічних значеннях рН-------------------------------------------------------------------------------------------------------------------------------------------------------------------------It is set that safety of erythrocytes of sheep in the conditions of hypertonic shock higher than safety of red erythrocytes of man at 0 о С, and below — at 37 о С. Thus the erythrocytes of sheep are more sensible to temperature to the terms during such stressing influence as hypertonic shock, by what erythrocytes of man. It can be related to different phospholipid composition of membranes of sheep and man.. It is show that the protective effect of butanol in relation to the erythrocytes of sheep is expressed identically at any explored value of pH environment; here safety of erythrocytes is increased from 40 % to 90 %. For the erythrocytes of man the protective effect of butanol is at that level and more expressed at the unphysiological values of pH. It is assumed that for both types of erythrocytes the protective effect of butanol at hypertonic shock probably can be related to protective influence on the proteins of cytoskeleton. In presence hexanol safety of erythrocytes of both kinds either is insignificantly increased or does not change. Absence of protective effect at hexanol in the conditions of hypertonic shock it is possible caused penetrating into a cell far slower, than butanol. Its co-operation with a cell is localized in the beginning at the level of membrane, that at hypertonic shock can not sufficient protective for a cell. At comparison of level of safety of cells in the conditions of hypertonic shock at the different values of pH it in absence of alconols, that safety of erythrocytes of sheep differs insignificantly at the different values of pH, while safety of red erythrocytes of man changes more considerably, diminishing at the unphysiological values of pH. It is explained by different proteins composition of membranes of man and sheep, and also possibly, by greater protected of proteins in the membrane of red erythrocytes of sheep, caused by the presence of ordered phospholipid structures.