Механізм захисної дії амфіфільних сполук в умовах гіпертонічного гемолізу еритроцитів

Abstract

Antihemolytic effect of various amphiphilic compounds under conditions of red blood cell hypertonic hemolysis at different temperatures (0 and 37 °C) and cell exposure to diamide was investigated. The level of maximum antihemolytic activity and values of efficient concentrations for all studied substances were lower at 0°C if compared with 37°C. Exposure of erythrocytes to diamide (5 and 10 mmol/l) did not change cell sensibility to hypertonic medium. There has been demonstrated a temperature-dependent decrease in the efficiency of studied substances under hypertonic hemolysis of erythrocytes exposed to 10 mmol/l diamide. Found reduction in efficiency of amphiphiles at low temperature and at high concentration of diamide was probably caused by similar changes of structural and dynamic state of erythrocyte membrane.