A study of antiulcer activity of cryoconserved placenta extract on the model of alcohol prednisolone-induced stomach lesions

Abstract

Search the new approaches to the treatment of peptic ulcer disease is an urgent problem of modern medicine. One of the potential antiulcer agents is cryopreserved placenta extract. The study was conducted on 28 male rats weighing 200–220 grams. After 24 hours of fasting, rats were administered intragastrically with prednisolone (20 mg/kg) dissolved in 80.0% ethyl alcohol (0.6 ml/100 grams of animal body weight). Cryopreserved placenta extract was administered intramuscularly at a dose of 0.16 ml/kg body weight in the prophylactic mode – once a day for 5 days before the introduction of alcohol-prednisolone mixture. 24 hours after administration of the alcohol-prednisolone mixture, rats were removed from the experiment and macroscopically assessed the condition of the gastric mucosa according to the following criteria: bloating, edema, redness, hemorrhage and folding disorders. For each group, the percentage of experimental animals was calculated according to the specified characteristics and the average value of their expression, which was evaluated on a scale: 0–3 points. The study showed that in 100.0% of control rats (model pathology without treatment) marked (3 [3; 3] points) hyperemia of the gastric mucosa (p<0,05). In addition, the presence of hemorrhage, edema and folding disorders caused by the introduction of alcohol-prednisolone mixture was noted. Prophylactic five-day administration of cryopreserved placenta extract before the introduction of ulcerogenic mixture led to a statistically significant (p<0.05) decrease in the severity of damage to the gastric mucosa in rats. Thus, hyperemia, hemorrhage and mild edema of the gastric mucosa were observed in only 28.6% of rats. The obtained data indicate the ability of cryopreserved placenta extract in the prophylactic mode of administration to increase the endurance of the gastric mucosa to the action of alcohol-prednisolone mixture.